Total views : 375

Evaluation of Anti-Asthmatic Activity of Centratherum anthelminticum Linn. in Experimental Animals


  • Department of Pharmacology, Sardar Patel College of Pharmacy, Bakrol - 388 315, Dist-Anand, Gujarat, India


Purpose: The aim of study was to evaluate the scientific basis for the traditional use of Centratherum anthelminticum Linn. seeds in asthma.

Design/Methodology: In the present study, methanolic extract of Centratherum anthelminticum seeds was evaluated for preliminary phytochemical study and antiasthmatic activity by Histamine induced preconvulsion dyspnoea (PCD), Mast cell degranulation (MCD) by compound 48/80 and Egg albumin induced asthma at different dose levels. In PCD animals were divided into three groups and % increase in time of PCD was recorded. In MCD, animals were divided into seven groups and % protection of mast cells was measured. In egg albumin induced asthma, animals were divided into five groups. Broncho alveolar fluid was collected and evaluated. Dunnett's t-test and post hoc test were used for statistical analysis.

Findings: Phytochemical study revealed the presence of flavonoids, proteins, Saponins, tannins, steroids, glycosides and phenols. The results of pharmacological studies showed that the methanolic extract of C.anthelminticum significantly decreased bronchospasm induced by histamine and protected the mass cell degranulation as compared to the control groups. It also significantly decreased the total leukocyte and different WBC count in egg albumin induced asthma.

Conclusions: Methanolic extract of C.anthelminticum seeds possess antiasthmatic activity. Antiasthmatic action of C.anthelminticum could be due to its antihistaminic, mast cell stabilizing property and preventive effect on infiltration of inflammatory cells.


Centratherum anthelminticum Linn. , Histamine Induced Preconvulsion Dyspnoea (CPD), Mast Cell Degranulation (MCD), Egg Albumin Induced Asthma, Compound 48/80.

Full Text:

 |  (PDF views: 114)


  • Behera D, Sehgal S. Bronchial asthma - Issues for the developing world. Indian journal of medical research. 2015;141: 380-382.
  • Masoli M, Fabian D, Holt S, Beasley R. global Initiative for Asthma (GINA) programme. The global burden of asthma: executive summary of the GINA Dissemination Committee report. Allergy. 2004; 59:469–78.
  • Stanojevic S, Moores G, Gershon AS, Bateman ED, Cruz AA. Global asthma prevalence in adults: findings from the cross-sectional world health survey. BMC Public Health. 2012; 12:204.
  • Agarwal R, DhooriaS et. al. Guidelines for diagnosis and management of bronchial asthma: Joint ICS/NCCP (I) recommendations. Lung India. 2015; 32: S3-S42.
  • Agarwal R, Denning D, Chakrabarti A. Estimation of the Burden of Chronic and Allergic Pulmonary Aspergillosis in India. PLoS ONE. 2014; 9(12): e114745.
  • Patil SS. Study of herbal formulation consisting of various indigenous plants for their anti-asthmatic activity in experimental animals. International Journal of Research in Ayurveda & Pharmacy. 2010; 1: 515-521.
  • Taur D, Patil R. Some medicinal plants with antiasthmatic potential: a current status. Asian pac J Trop Biomed. 2011; 1: 413-418.
  • Kirtikar KR, Basu BD. Indian medicinal plants. Dehradun: International Book Distributor. 1999, p. 2267- 70.
  • Qureshi S, Rais S, Usmani R et. al. Centratherum anthelminticum seeds reverse the carbon tetrachlorideinduced hepatotoxicity in rats. African journal of pharmacy and pharmacology. 2016; 10:533-539.
  • Purnima, A.,. Koti B.C,. Tikare V.P, Viswanathaswamy A.H., Thippeswamy A.H. and Dabadi P.. Evaluation of analgesic and antipyretic activities of Centratherum anthelminticum (L) Kuntze seed. Indian J. Pharm. Sci. 2009; 71: 461-464.
  • Iqbal, Z.,. Lateef M,. Jabbar A,. Akhtar M.S and Khan M.N.. Anthelmintic activity of Vernonia anthelmintica seeds against trichostrongylid nematodes of sheep. Pharm. Biol. 2006; 44: 563-567.
  • Nisha M.,. Kalyanasundaram M, Paily K.P.A., Vanamail P.and Balaraman K.. In vitro screening of medicinal plant extracts for macrofilaricidal activity. Parasitol. Res. 2007; 100: 575-579.
  • Arya, A., Mohd A.M., Achoui M., Cheah S.C. and Mohan S.. Chloroform fraction of Centratherum anthelminticum (L.) seed inhibits tumor necrosis factor alpha and exhibits pleotropic bioactivities: Inhibitory role in human tumor cells. Evidence-Based Complement. Altern. Med. 2012; 10.1155/2012/627256.
  • Ani, V., Naidu K.A.. Antihyperglycemic activity of polyphenolic components of black/bitter cumin Centratherum anthelminticum (L.) Kuntze seeds. Eur. Food Res. Technol. 2008; 226: 897-903.
  • Zho U,. Shang J., J, Ping F.and Zhao G.. Alcohol extract from Vernoniaanthelmintica (L.) willd seed enhances melanin synthesis through activation of the p38 MAPK signaling pathway in B16F10 cells and primary melanocytes. J. Ethnopharmacol. 2012; 143: 639-647.
  • Saxena P, Saxena P. In-vitro and in-vivo Evaluation of Anti Asthmatic Activity of Rhizomes Extract of AcorusCalamus (Linn.) in Guinea Pigs. Res. J. Pharmaceutical Sci. 2014; 3: 1-6.
  • Sagar R, Sahoo H. Evaluation of antiasthmatic activity of ethanolic extract of Elephanto-pusscaber L. leaves. Indian journal of pharmacology. 2012; 44: 398-401.
  • Patel J, Shah S, Deshpande S, Shah G. Evaluation of the antiasthmatic activity of leaves of Vitex negundo. Asian journal of Pharmaceutical and Clinical research. 2009; 1: 81-86.
  • Mitra S.K., S. Gopumadhvan, M.V. Venkataranganna and S.D. Anturliker. Anti-asthmatic and Anti anaphylactic effect of e-721B, a polyhedral formulastion. Ind.J. Pharmacol. 1993; 31:133-137.
  • Nelson HS. Prospects for antihistamines I the treatment of asthma Allergy. Clinmmunl. 2003; 112:96-100.
  • Sagar R, Sahoo H. Pharmacological evaluation of calendula officinalis L. on bronchial asthma in various experimental animals. International Journal of Nutrition, Pharmacology, Neurolo-gical Diseases. 2014; 4:95-103.
  • Raju A, Unger B et. al. Evaluation of anti asthmatic activity of hydro alcoholic extract of Citrullus colocynths and Cucumistri gonus fruits. Journal of Applied Pharmaceutical Science. 2015; 05:126-130.